FGF Receptor 1 Monoclonal Antibody
Catalog No.: 30102
Clone No.: M5G10
Format: 250ug Protein G-purified antibody in PBS, pH 7.4
The fibroblast growth factor (FGF) family is a group of multi-functional polypeptides that function in embryonic development, tissue repair, neuronal cell survival, cardiovascular disease, and tumorigenesis. The biological activities of FGF are mediated by cell surface receptors that have tyrosine kinase activity. FGFR-1 is activated by FGF-1 and FGF-2. In neoplastic cells, overexpression of FGF-1, FGF-2 and FGF receptors appear to contribute to cellular trans- formation and proliferation.
Antigen: Recombinant human ectodomain of FGFR-1β expressed in E. coli beginning with proline 23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Host Species: Mouse Antibody Subclass: IgG1 Accession no.: P11362
Specificity: This antibody reacts with both α (denatured) and β isoforms. Epitope is within the sequence his241 and val267 between Ig loops II and III. Epitope is masked in undenatured FGFr1α.
Immunohistochemistry: 10ug/ml, paraffin- embedded tissue.
Immunoblotting: 1ug/ml recognizes 10ng recombinant bacterial, baculoviral or native FGFR1.
Immunoprecipitation: native, recombinant, and ligand-labeled FGFr1.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Xu et al. (1992) J Biol Chem 267: 17792
Wang et al. (1995) J Biol Chem 270: 10231
Myoken et al. (1996) J Pathol 178: 429
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.