FGF Receptor 1α Monoclonal Antibodies
Catalog No.: 30105 (M1C4) Size: 250 ug Protein G-purified antibody in PBS, pH 7.4
Antigen: Recombinant human ectodomain of FGFr1α expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly- ile and COOH-terminal glu-phe sequences.
Host Species: Mouse
Antibody Class: IgG1
Preservatives: None. Available on request.
30105 reacts with the COOH-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu75 and ser119 of FGFr1α. Epitope is masked in undenatured FGFr1α.
Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculo- viral, or native FGFr1.
Immunoprecipitation: Ig loop I fragment of denatured, intact FGFr1α.
Immunohistochemistry: untested; predicted to be poor due to masked epitope.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Xu et al. (1992) J Biol Chem 267: 17792.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.