Anti-Fibroblast Growth Factor Receptor 1α (FGFR1α) Antibody (30104)

$596.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 250ug WB,IHC,IP,FACS Human, Mouse, Rat
SKU: 30104 Categories: , ,
Overview
Product Name Anti-Fibroblast Growth Factor Receptor 1α (FGFR1α) Antibody (30104)
Description Anti-FGF Receptor-α Mouse Monoclonal Antibody
Target Fibroblast Growth Factor Receptor 1α (FGFR1α)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,IP,FACS
Host Mouse
Clonality Monoclonal
Clone ID M2F12
Isotype IgG2a
Immunogen Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH- terminal glu-phe sequences.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity 30104 reacts with the NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1alpha. Reacts with human, rat, and mouse receptor. Other species untested.
Target Name Fibroblast growth factor receptor 1
Target ID Fibroblast Growth Factor Receptor 1α (FGFR1α)
Uniprot ID P11362
Alternative Names FGFR-1, EC 2.7.10.1, Basic fibroblast growth factor receptor 1, BFGFR, bFGF-R-1, Fms-like tyrosine kinase 2, FLT-2, N-sam, Proto-oncogene c-Fgr, CD antigen CD331
Gene Name FGFR1
Sequence Location Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle. Note=After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus.
Biological Function Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation. {UniProtKB:P16092, PubMed:10830168, PubMed:11353842, PubMed:12181353, PubMed:1379697, PubMed:1379698, PubMed:15117958, PubMed:16597617, PubMed:17311277, PubMed:17623664, PubMed:18480409, PubMed:19224897, PubMed:19261810, PubMed:19665973, PubMed:20133753, PubMed:20139426, PubMed:21765395, PubMed:8622701, PubMed:8663044}.
Research Areas Growth Factors, Cytokines, Receptors
Application Images
Description Immunohistochemical staining of growth factors and quantitative analysis in aortic plaques from high cholesterol-fed rabbits.(A) Protein expression of vascular endothelial growth factor A (VEGF-A), VEGF receptor 2 (VEGFR-2), basic fibroblast growth factor 2 (FGF-2), FGF receptor 1 (FGFR)-1, platelet-derived growth factor-BB (PDGF-BB) and PDGF receptor Beta (PDGFR-Beta). (Bars = 50 μm). (B–F) Quantification of VEGF-A, VEGFR-2, FGF-2, FGFR-1, PDGF-BB, and PDGFR-Beta. Data are presented as the mean±SEM. *p<0.05 vs. week 4; #p<0.05 vs. week 6; ^p<0.05 vs. week 8; &p<0.05 vs. week 10.
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculo-viral, or native FGFr1.

Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1.

Immunohistochemistry: frozen sections of human glioblastomas, para-formaldehyde- fixed cells and nuclei, human myocardial biopsies.
References & Data Sheet
References Xu et al. (1992) J Biol Chem 267: 17792; ; Proudovsky et al. (1994) J Biol Chem 269: 31720; ; Morrison et al. (1994) Cancer Res 54: 2794; ; Zhao et al. (1994) J Clin Invest 94: 992; ; Feng et al. (1996) Biochim Biophys Acta 1310: 67.; Mao, Y., Liu, X., et al.(2018) PLoS One. 20;13(8):e0201395 ; VEGF-A/VEGFR-2 and FGF-2/FGFR-1 but not PDGF-BB/PDGFR-β play important roles in promoting immature and inflammatory intraplaque angiogenesis. PLoS One (2018) [30125282]