Anti-Fen-1 Antibody (56208)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,IP Human
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Overview
Product Name Anti-Fen-1 Antibody (56208)
Description Anti-Fen-1 Mouse Monoclonal Antibody
Target Fen-1
Species Reactivity Human
Applications WB,ICC/IF,IP
Host Mouse
Clonality Monoclonal
Clone ID 4.00E+07
Isotype IgG1
Immunogen Recombinant human FEN-1 protein (aa 1-380) purified from baculovirus-infected Sf-9 cells.
Properties
Form Liquid
Concentration Lot Specific
Formulation 10 mM PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody recognizes human FEN-1.
Target Name Flap endonuclease 1
Target ID Fen-1
Uniprot ID P39748
Alternative Names FEN-1, EC 3.1.-.-, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1
Gene Name FEN1
Sequence Location [Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion
Biological Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. {HAMAP-Rule:MF_03140, PubMed:10744741, PubMed:11986308, PubMed:18443037, PubMed:20729856, PubMed:26751069, PubMed:7961795, PubMed:8621570}.
Research Areas Neuroscience
Background FEN-1 (Flap Endonuclease and Five Prime Exonuclease) is a 42 kD protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 appears to have important roles in DNA replication and repair and is required in cell-fre DNA replication systems.
Application Images
Description Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody (56208) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description Immunofluorescence analysis of paraformaldehyde-fixed MCF7, using FEN1 [4E7](56208) antibody at 1:200 dilution.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5 ug/mL.

Positive controls: MCF-7 cells and recombinant fusion protein.
References & Data Sheet