qed-bioscience-anti-fen-1-antibody-56208-1

qed-bioscience-anti-fen-1-antibody-56208-2

Anti-Fen-1 Antibody

$461.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Mouse	100ug	WB,ICC/IF,IP	Human

SKU: 56208 Categories: Antibody Products, Neuroscience and Signal Transduction Antibodies, Products

Overview

Product Name Anti-Fen-1 Antibody (56208)

Description Anti-Fen-1 Mouse Monoclonal Antibody

Target Fen-1

Species Reactivity Human

Applications WB,ICC/IF,IP

Host Mouse

Clonality Monoclonal

Clone ID 4.00E+07

Isotype IgG1

Immunogen Recombinant human FEN-1 protein (aa 1-380) purified from baculovirus-infected Sf-9 cells.

Properties

Form Liquid

Concentration Lot Specific

Formulation 10 mM PBS, pH 7.4.

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by Protein A affinity chromatography

Specificity This antibody recognizes human FEN-1.

Target Name Flap endonuclease 1

Target ID Fen-1

Uniprot ID P39748

Alternative Names FEN-1, EC 3.1.-.-, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1

Gene Name FEN1

Sequence Location [Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion

Biological Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. {HAMAP-Rule:MF_03140, PubMed:10744741, PubMed:11986308, PubMed:18443037, PubMed:20729856, PubMed:26751069, PubMed:7961795, PubMed:8621570}.

Research Areas Neuroscience

Background FEN-1 (Flap Endonuclease and Five Prime Exonuclease) is a 42 kD protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 appears to have important roles in DNA replication and repair and is required in cell-fre DNA replication systems.

Description Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody (56208) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.