Awaiting product image

Anti-FAK (Phospho-Tyr861) Antibody (43059)

$355.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB Human
SKU: 43059 Categories: ,
Overview
Product Name Anti-FAK (Phospho-Tyr861) Antibody (43059)
Description Anti-FAK (Phospho-Tyr861) Rabbit Polyclonal Antibody
Target FAK (Phospho-Tyr861)
Species Reactivity Human
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of tyrosine 861 (H-I-Y(p)-Q-P) derived from human FAK.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human FAK only when phosphorylated at tyrosine 861.
Target ID FAK (Phospho-Tyr861)
Uniprot ID Q05397
Post Translational Modification FAK (Phospho-Tyr861)
Target Phospho-tyr861
Research Areas Phosphospecific Antibodies
Background Focal Adhesion Kinase, FAK (also known as PTK2 protein tyrosine kinase 2, PTK2) is a protein that, in humans, is encoded by the PTK2 gene. FAK is a focal adhesion-associated protein kinase involved in cellular adhesion and spreading processes. It has been shown that when FAK is blocked, breast cancer cells become less metastatic due to decreased mobility.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~125kDa is detected.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet