Anti-F1Aa Antibody (2279)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human, Mouse, Rat
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Overview
Product Name Anti-F1Aa Antibody (2279)
Description Anti-F1Aα (CT) Rabbit Polyclonal Antibody
Target F1Aa
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 609- 622 of human F1A (accession no. AAF05314). This sequence is identical to that of mouse.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat F1A (70kDa).
Target Name Protein fem-1 homolog B
Target ID F1Aa
Uniprot ID Q9UK73
Alternative Names FEM1b, FEM1-β, Fem-1-like death receptor-binding proteinα, Fem-1-like in apoptotic pathway proteinα, F1A-α
Gene Name FEM1B
Gene ID 10116
Accession Number NP_056137
Sequence Location Cytoplasm, Nucleus
Biological Function Substrate-rUniProtKB:Q9Z2G0, PubMed:10542291, PubMed:19330022, PubMed:24076122, PubMed:28118078, PubMed:29779948, PubMed:33398168, PubMed:33398170}.
Research Areas Apoptosis
Background Fas and tumor necrosis factor receptor 1 (TNFR1) are two members of the family of death receptors. A novel protein that associates with the intracellular domains of Fas and TNFR1 has been designated image (or FEM1 ). F1A is cleaved by CED-3 and caspase, and it associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1A induces apoptosis. F1A is expressed in a variety of human and mouse tissues.
Application Images
Description Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 ug/mL.
Description Immunohistochemistry of F1Aα in mouse liver tissue with F1Aα antibody at 5 ug/mL.
Description Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 ug/mL.
Description Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 ug/ml. Green: F1A alpha Antibody (2279)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunohistochemistry: use at 5ug/mL.

Positive control: Mouse liver tissue lysate.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet