Anti-Estrogen Receptor β Antibody (3336)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,ChIP,IHC Human
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Overview
Product Name Anti-Estrogen Receptor β Antibody (3336)
Description Anti-Estrogen Receptor β Mouse Monoclonal Antibody
Target Estrogen Receptor β
Species Reactivity Human
Applications WB,ICC/IF,ChIP,IHC
Host Mouse
Clonality Monoclonal
Clone ID 6A12
Isotype IgG2b
Immunogen 6-His fusion protein containing the region encoding aa 1-153 of human estrogen receptor-b (ER-b) expressed in E. coli.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human ER-b, a novel receptor (approx. 52 kD) recently isolated from testis. The size and structure of ER-b are very similar to ER-alpha with the ligand and DNA binding domains being highly conserved. The N-terminus of each receptor, which serves as their transactivation domain, are significantly different. Like ER-alpha, ER-b binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements.
Target Name Estrogen receptor β
Target ID Estrogen Receptor β
Uniprot ID Q92731
Alternative Names ER-β, Nuclear receptor subfamily 3 group A member 2
Gene Name ESR2
Gene ID 2100
Accession Number NP_001035365
Sequence Location Nucleus
Biological Function Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). {PubMed:20074560, PubMed:29261182, PubMed:30113650, PubMed:9325313}.; [Isoform 2]: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. {PubMed:9671811}.
Research Areas Cancer Research
Application Images
Description Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12] (3336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5 ug/mL.

Positive controls: Native receptor from human thymus, spleen, ovary, and testis or recombinant protein.
References & Data Sheet