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Anti-Estrogen Receptor-a (Phospho-Ser167) Antibody (43073)

$355.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC,IF Human
SKU: 43073 Categories: , ,
Overview
Product Name Anti-Estrogen Receptor-a (Phospho-Ser167) Antibody (43073)
Description Anti-Estrogen Receptor-a (Phospho-Ser167) Rabbit Polyclonal Antibody
Target Estrogen Receptor-a (Phospho-Ser167)
Species Reactivity Human
Applications WB,IHC,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 167 (L-A-S(p)-T-N) derived from human estrogen receptor-a and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human estrogen receptor-alpha only when phosphorylated at serine 167.Accession no.: P03372, NP_000116.2
Target Name Estrogen receptor a
Target ID Estrogen Receptor-a (Phospho-Ser167)
Uniprot ID G1E6Z3
Gene Name Era
Post Translational Modification Estrogen Receptor-a (Phospho-Ser167)
Target Phospho-ser167
Research Areas Phosphospecific Antibodies
Background Estrogen receptor alpha (ERalpha), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is one of two main types of estrogen receptor, a nuclear receptor that is activated by estrogen. In humans, ERalpha is encoded by the gene ESR1 (Estrogen Receptor 1). Estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~66kDa is detected.

Immunofluorescence: use at dilution of 1:100-1:200.

Immunohistochemistry: use at dilution of 1:50-1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet