Estrogen Receptor-α (Phospho-Ser104)

Estrogen Receptor-α (Phospho-Ser104) Polyclonal Antibody

ORDERING INFORMATION

Catalog No.: 43070
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.

BACKGROUND

Estrogen receptor alpha (ERα), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is one of two main types of estrogen receptor, a nuclear receptor that is activated by estrogen. In humans, ERα is encoded by the gene ESR1 (Estrogen Receptor 1). Estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.

SPECIFICATION SUMMARY

Antigen: Peptide sequence that includes phosphorylation site of serine 104 (S-V-S(p)-P-S) derived from human estrogen receptor and conjugated to KLH.
Host Species: Rabbit
Specificity: This antibody detects endogenous human estrogen receptor only when phosphorylated at serine 104.
Accession no.: P03372, NP_000116.2

APPLICATIONS

Immunoblotting: use at dilution of 1:500- 1:1,000. A band of ~66kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100.
These are recommended working dilutions. Enduser should determine optimal dilutions for their application.

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term.

For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.

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