Entamoeba histolytica

Entamoeba histolytica Monoclonal Antibodies

ORDERING INFORMATION

Catalog No. 15000	Clone No. EH34.5	MAb Subtype IgG1*	Size 100ug, 500ug	Library Pack No. 150101	100ug/clone All 5 clones
15001	EH123.5	IgG1*	100ug, 500ug
15010	EH147.5	IgM**	100ug, 500ug
15020	EH304.6	IgG3**	100ug, 500ug
15030	EH357.8	IgM**	100ug, 500ug

Format: *Protein G-purified antibody in PBS, pH 7.4
**PEG-purified antibody in PBS, pH 7.4

BACKGROUND

Entamoeba histolytica is an anaerobic parasitic protozoan that predominantly infects humans and other primates causing amoebiasis. In the vast majority of cases, infection is asymptomatic and the carrier is unaware they are infected. However, in an estimated 10% of cases E. histolytica causes disease. Once the trophozoites are excysted they colonize the large bowel, remaining on the surface of the mucus layer and feeding on bacteria and food particles. Occasionally, and in response to unknown stimuli, trophozoites move through the mucus layer where they come in contact with the epithelial cell layer and start the pathological process.

SPECIFICATION SUMMARY

Antigen: Entamoeba histolytica HK-9.
Host Species: Mouse
Specificity: These antibodies recognize Entamoeba histolytica trophozoites and cysts.

APPLICATIONS

These antibodies have been qualified for use in ELISA to detect E. histolytica trophozoites and cysts. Endusers should determine optimal concentrations for their applications.

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

These antibodies are stable for at least one (1) year at -20° to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.

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