Anti-ENL Monoclonal Antibody
Catalog no.: 34043
Size: 100ug Protein G-purified antibody in PBS, pH 7.4.
The AF4 and ENL (Eleven nineteen Leukemia) protein families are the most frequent MLL fusion partners accounting for two-thirds of MLL-associated leukemia incidence. The ENL family includes ENL and AF9 and has structural homology to the yeast Anc1 protein. ENL is a nuclear protein that is capable of activating transcription from synthetic reporter genes in both lymphoid and myeloid cells as well as in yeast. Deletion muta-genesis demonstrated that the minimal portion of ENL required for activation of transcription was localized to its C-terminal 90 amino acids. This region is highly conserved between ENL and AF9 and is retained in all Hrx-ENL and Hrx-AF9 fusion proteins. Thus, the leukemogenic contribution and transcriptional activation potential of ENL colocalize to its highly conserved carboxy terminus.
Antigen: Maltose-binding fusion protein containing ENL amino acids 414-472 (Accession no. NP_005925).
Clone no.: 3.1.1
Antibody Class: IgG1
Preservatives: None – available on request.
This antibody recognizes human ENL.
Immunoblotting: use at 1-5ug/ml. A band of ~62kDa is detected.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Rubnitz JE et al. 1994 Blood 84: 1747-1752.
Yokoyama A et al. 2010 Cancer Cell 17: 198-212.
Mueller D at al. 2007 Blood 110: 4445-4454.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.