Product Name Anti-ENL Antibody (34043)
Description Anti-ENL Mouse Monoclonal Antibody
Species Reactivity Human
Clone ID 3.1.1
Immunogen Maltose-binding fusion protein containing ENL amino acids 414-472 (Accession no. NP_005925).
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Purification Purified by Protein G affinity chromatography
Specificity This antibody recognizes human ENL.
Target Name Protein ENL
Target ID ENL
Uniprot ID Q03111
Alternative Names YEATS domain-containing protein 1
Gene Name MLLT1
Sequence Location Nucleus.
Biological Function Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA (PubMed:20159561, PubMed:20471948). Specifically rPubMed:20159561, PubMed:20471948, PubMed:27105114}.; Acts as a key chromatin reader in acute myeloid leukemia by rPubMed:28241139, PubMed:28241141}.
Research Areas Cancer research
Background The AF4 and ENL (Eleven nineteen Leukemia) protein families are the most frequent MLL fusion partners accounting for two-thirds of MLL-associated leukemia incidence. The ENL family includes ENL and AF9 and has structural homology to the yeast Anc1 protein. ENL is a nuclear protein that is capable of activating transcription from synthetic reporter genes in both lymphoid and myeloid cells as well as in yeast. Deletion muta-genesis demonstrated that the minimal portion of ENL required for activation of transcription was localized to its C-terminal 90 amino acids. This region is highly conserved between ENL and AF9 and is retained in all Hrx-ENL and Hrx-AF9 fusion proteins. Thus, the leukemogenic contribution and transcriptional activation potential of ENL colocalize to its highly conserved carboxy terminus.
Description Immunoblotting: use at 1-5ug/ml. A band of ~62kDa is detected. Extracts from transduced 293fENL and HEK293 parental cells blotted with #34043.
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5ug/mL. A band of ~62kDa is detected.