Anti-EndoPDI Antibody (56458)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP,ELISA Human
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Overview
Product Name Anti-EndoPDI Antibody (56458)
Description Anti-EndoPDI Mouse Monoclonal Antibody
Target EndoPDI
Species Reactivity Human
Applications WB,IHC,ICC/IF,IP,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 2E7/7
Isotype IgG2a
Immunogen Synthetic peptide corresponding to aa 52-63 (ADGEDGQDPHSK) of human EndoPDI (accession no. NP_001139021).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human EndoPDI.
Target Name Thioredoxin domain-containing protein 5
Target ID EndoPDI
Uniprot ID Q8NBS9
Alternative Names Endoplasmic reticulum resident protein 46, ER protein 46, ERp46, Thioredoxin-like protein p46
Gene Name TXNDC5
Sequence Location Endoplasmic reticulum lumen
Biological Function Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast (By similarity). {ECO:0000250}.
Research Areas Neuroscience
Background Endothelial protein disulfide isomerase (EndoPDI) is a thiredoxin member of the protein disulfide isomerase family of chaperones. This enzyme is localized in the endoplasmic reticulum in primary endothelial cells but colocalizes with nucleoli in the nuclei of breast, colon, and kidney cancer cells. EndoPDI mRNA and protein expression is iduced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EndoPDI Monoclonal Antibody, Clone 2E7/7 (56458). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody (56458) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~48kDa is detected.

Positive control: heat-shocked HeLa cell lysate.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet