Catalog No.: 11004
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Antigen: Peptide corresponding to aa 55- 70 of human EndoG (accession no. NP_004426).
Host Species: Rabbit
Preservatives: 0.02% sodium azide
This antibody recognizes human, mouse, and rat EndoG (Endonuclease G) an enzyme that is activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondria-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis.
Immunoblotting: use at 1-2ug/ml. A band of 35kDa is detected.
Immunohistochemistry: use at 10-15ug/ml.
Positive control: HepG2 cell lysate.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.