Elk1 (Phospho-Ser389) Polyclonal Antibody
Catalog No.: 43037
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Antigen: Peptide sequence that includes phosphorylation site of serine 389 (P-R-S(p)-P-A) derived from human Elk1 and conjugated to KLH.
Accession no.: P19419, NP_001107595.1
Host Species: Rabbit
Specificity: This antibody detects endogenous human, mouse, and rat Elk1 only when phosphorylated at serine 389.
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~62kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term use.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.