ECS (DDDDK) Affinity-Purified Polyclonal Antibody
Catalog No., Size
Format: 1mg/ml in Phosphate Buffered Saline, pH 7.2, 0.09% sodium azide.
Enterokinase, also known as enteropeptidase, is a serine protease that catalyzes the hydrolysis of peptide bonds in proteins but, unlike other kinases, does not catalyze transfer of phosphate groups. Enterokinase cleaves proteins following a lysine at an enterokinase cleavage site, or ECS (Asp-Asp-Asp-Asp-Lys). In vivo, this cleavage results in trypsin-dependent activation of other pancreatic zymogens, such as chymotrypsinogen, proelastase, procarboxypeptidase and prolipase in the lumen of the gut.
Antigen: DDDDK conjugated to KLH.
Host Species: Goat
Specificity: Antibody was affinity-purified on the peptide.
Immunoblotting: use at a dilution of 1:1,000- 1:20,000.
Immunocytochemistry: use at a dilution of 1:100-1:400.
ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500.
Immunoprecipitation: use 1-4ug/mg lysate.
These are recommended dilutions. Endusers should determine optimal dilutions for their applications. Detection of ECS-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18830 diluted 1:25,000.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at 2-8°C.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.