ECS (DDDDK) Affinity-Purified Polyclonal Antibody, HRP Conjugate
Catalog No., Size
Format: 1mg/ml conjugated to horseradish peroxidase (HRP) in Phosphate Buffered Saline (PBS), 0.1% Pro-Clean 400. Molar enzyme/antibody ratio = 4:1.
Enterokinase, also known as enteropeptidase, is a serine protease that catalyzes the hydrolysis of peptide bonds in proteins but, unlike other kinases, does not catalyze transfer of phosphate groups. Enterokinase cleaves proteins following a lysine at an enterokinase cleavage site, or ECS (Asp-Asp-Asp-Asp-Lys). In vivo, this cleavage results in trypsin-dependent activation of other pancreatic zymogens, such as chymotrypsinogen, proelastase, procarboxypeptidase and prolipase in the lumen of the gut.
Antigen: DDDDK conjugated to KLH.
Host Species: Rabbit
Specificity: Antibody was affinity-purified on the peptide.
Immunoblotting: use at a dilution of 1:1,000- 1:30,000.
Immunocytochemistry: use at a dilution of 1:200-1:500.
ELISA: use at a dilution of 1:10,000- 1:100,000.
These are recommended dilutions. Endusers should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at 2-8°C.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.