Anti-DRAK1 (NT) Antibody (2147)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse, Rat
SKU: 2147 Categories: , ,
Overview
Product Name Anti-DRAK1 (NT) Antibody (2147)
Description Anti-DRAK1 (NT) Rabbit Polyclonal Antibody
Target DRAK1 (NT)
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 5-19 of human DRAK1 (accession no. Q9UEE5).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human DRAK1 (50kDa). No cross-reactivity with DRAK2, DAP or ZIP kinases.
Target Name Serine/threonine-protein kinase 17A
Target ID DRAK1 (NT)
Uniprot ID Q9UEE5
Alternative Names EC 2.7.11.1, DAP kinase-related apoptosis-inducing protein kinase 1
Gene Name STK17A
Gene ID 9263
Accession Number NP_004751.2
Sequence Location Nucleus
Biological Function Acts as a positive regulator of apoptosis. Also acts as a regulator of cellular reactive oxygen species. {PubMed:21489989, PubMed:9786912}.
Research Areas Apoptosis
Background Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis have been identified and designated DRAK1 and DRAK2 for DAP kinase–related apoptosis-inducing protein kinases. DRAKs contain an N-terminal kinase domain and a C- terminal regulation domain. Overexpression of DRAK1 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases which mediate apoptosis through their catalytic activities. DRAK1 is located in cell nuclei, and the mRNA for DRAK1 is ubiquitously expressed in human tissues.
Application Images
Description Western blot analysis of DRAK1 in (A) MOLT4 and (B) A431 whole cell lysates with DRAK1 antibody at 1 ug/mL.
Description Immunocytochemistry staining of MOLT4 cells using DRAK1 antibody at 2 ug/mL.
Description Immunofluorescence of DRAK1 in Molt cells with DRAK1 antibody at 20 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1ug/mL.

Immunocytochemistry: use at 2ug/mL.

Positive control: Whole cell lysate from A431 or MOLT4 cells.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet