Catalog No.: 2421
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain-containing receptors. TRAIL/Apo2L is a new member of the TNF family. A novel death domain- containing receptor for TRAIL was recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several independent laboratories. Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL- induced cell death. Overexpression of DR5 induces apoptosis and activates NF- B.
Antigen: Peptide corresponding to aa 388- 407 of human DR5 precursor (accession no. AF012535).
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes full-length human and mouse DR5 (57kDa).
Immunoblotting : use at 2ug/ml.
Immunocytochemistry: use at 5ug/ml.
Positive control: Whole cell lysate from HeLa cells or K562 cells.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.