Anti-DOG1 Antibody (34036)

$400.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug IHC Human
SKU: 34036 Categories: , ,
Overview
Product Name Anti-DOG1 Antibody (34036)
Description Anti-DOG1 Mouse Monoclonal Antibody
Target DOG1
Species Reactivity Human
Applications IHC
Host Mouse
Clonality Monoclonal
Clone ID DOG1.1
Isotype IgG1
Immunogen Synthetic peptide corresponding to aa 904-986 of human DOG1 (accession no. Q5XXA6).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human DOG1.
Target Name Anoctamin-1
Target ID DOG1
Uniprot ID Q5XXA6
Alternative Names Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2
Gene Name ANO1
Sequence Location Cell membrane, Cytoplasm
Biological Function Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. {PubMed:20056604, PubMed:21984732, PubMed:22178883, PubMed:22946059}.
Research Areas Cancer research
Background DOG1 (Discovered on GIST1), also known as TMEM16A and ANO1, has emerged in recent years as a promising biomarker for gastrointestinal stromal tumors (GIST). It was originally discovered through microarray expression profiling analysis as a gene that is highly expressed in GIST, and subsequent immunohistochemical studies have shown its use in its diagnosis. DOG1 antibodies are more sensitive than KIT antibodies in detecting tumors of gastric origin, tumors with epithelioid morphology, and tumors harboring PDGFRA mutation. DOG1 immunoreactivity is rarely observed in other mesenchymal and nonmesenchymal tumor types. These results support the use of DOG1 as a biomarker for GIST.
Application Images
Description Immunohistochemistry: Use at 110ug/ml on paraffin-embedded samples. Antigen retrieval solution: 10mM citrate, pH 6 (boil slides by microwaving in antigen retrieval solution for 12 minutes). r Host Species: Mouse Antibody Class: IgG1 Preservatives: None. Available on equest. BACKGROUND DOG1 (Discovered on GIST1), also known as TMEM16A and ANO1, has emerged in recent years as a promising biomarker for gastrointestinal stromal tumors (GIST). It was originally discovered through microarray expression profiling analysis as a gene that is highly expressed in GIST, and subsequent immunohistochemical studies have shown its use in its diagnosis. DOG1 antibodies are more sensitive than KIT antibodies in detecting tumors of gastric origin, tumors with epithelioid morphology, and tumors harboring PDGFRA mutation. DOG1 immunoreactivity is rarely observed in other mesenchymal and nonmesenchymal tumor types. These results support the use of DOG1 as a biomarker for GIST. SPECIFICITY This antibody recognizes human DOG1. QED Bioscience, Inc. 10919 Technology Place, Suite C San Diego, CA 92127 Membranous staining of DOG1 in GIST
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry: Use at 1- 10ug/ml on paraffin-embedded samples. Antigen retrieval solution: 10mM citrate, pH 6 (boil slides by microwaving in antigen retrieval solution for 12 minutes).User should determine optimal concentrations for their applications.
References & Data Sheet
References Espinosa I et al. 2008 Am J Surg Pathol 32: 210-218. ; Lee CH et al. 2010 Adv Anat Pathol 17: 222-232.