DOG1 Monoclonal Antibody
Catalog No.: 34036
Size: 100 ug Protein G-purified antibody in PBS, pH 7.4.
Antigen: Synthetic peptide corresponding to aa 904-986 of human DOG1 (accession no. Q5XXA6).
Clone no.: DOG1.1
Host Species: Mouse
Antibody Class: IgG1
Preservatives: None. Available on request.
DOG1 (Discovered on GIST1), also known as TMEM16A and ANO1, has emerged in recent years as a promising biomarker for gastrointestinal stromal tumors (GIST). It was originally discovered through microarray expression profiling analysis as a gene that is highly expressed in GIST, and subsequent immunohistochemical studies have shown its use in its diagnosis. DOG1 antibodies are more sensitive than KIT antibodies in detecting tumors of gastric origin, tumors with epithelioid morphology, and tumors harboring PDGFRA mutation. DOG1 immunoreactivity is rarely observed in other mesenchymal and nonmesenchymal tumor types. These results support the use of DOG1 as a biomarker for GIST.
This antibody recognizes human DOG1.
Immunohistochemistry: Use at 1- 10ug/ml on paraffin-embedded samples. Antigen retrieval solution: 10mM citrate, pH 6 (boil slides by microwaving in antigen retrieval solution for 12 minutes).
User should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Espinosa I et al. 2008 Am J Surg Pathol 32: 210-218. Lee CH et al. 2010 Adv Anat Pathol 17: 222-232.
For in vitro investigational use only. Not for use in diagnostic or therapeutic procedures.