Product Name Anti-DNA/RNA Oxidative Damage Antibody (12501)
Description Anti-DNA and RNA Oxidative Damage Markers Mouse Monoclonal Antibody
Target DNA/RNA Oxidative Damage
Species Reactivity Eukaryotic
Clone ID 15A3
Immunogen 8-hydroxyguanosine (oh8G)-BSA and â€“casein conjugates.
Concentration Lot Specific
Formulation Tris-buffered saline (TBS), pH 7.4
Buffer Formulation Tris
Buffer pH pH 7.4
Purification Purified by Protein A affinity chromatography
Specificity This antibody recognizes 8-hydroxy-2'-deoxyguanosine (oh8dG), 8-hydroxyguanine (oh8G), and 8-hydroxyguanosine (oh8G). These are markers of oxidative damage to DNA and RNA.
Target ID DNA/RNA Oxidative Damage
Research Areas DNA
Description Distribution of 8-oxoG in (A) H2O2-treated Chlamydomonas and (B) Arsenite-treated HeLa cells. Courtesy of William Zerges, Concordia University.(reference #6 below)
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in TBS or medium that is identical to that used in the assay system.
Application Instructions This antibody may be used in ELISA and immunohistochemistry to detect the markers of oxidative damage to DNA and RNA. May also be used on immunoaffinity columns to isolate oxidative DNA damage adducts from biological fluids.
References & Data Sheet
References Park et al. (1992) Assay of excised oxidative DNA lesions: Isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column. Proc Natl Acad Sci (USA) 89: 3375- 3379. Nunomura et al. (1999) RNA Oxidation is a prominent feature of vulnerable neurons in Alzheimer's Disease. J Neuroscience 19: 1959-1964. Cui et al. (1999) Oxidative damage to the c-fos gene and reduction of its transcription after focal cerebral ischemia. J Neurochemistry 73:1164-1174. Salganik et al. (2000) Dietary antioxidant depletion: enhancement of tumor apoptosis and inhibition of brain tumor growth in transgenic mice. Carcinogenesis 21: 909-914. Tanaka et al. (2007) Oxidized messenger RNA induces translation errors. Proc Natl Acad Sci (USA) 104: 66-71. Zhan et al. (2015) Localized control of oxidized RNA. Journal of Cell Science 128: 4210-4219. Kharel et al. (2016) Evidence of extensive RNA oxidation in normal appearing cortex of multiple sclerosis brain. Neurochemistry International 92: 43-48.
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