DNA Ligase III Monoclonal Antibody
Catalog No.: 56250 (clone 1F3)
Format: 100ug (1mg/ml) in PBS, pH 7.4. Purified from hybridoma culture supernatant by Protein G affinity chromatography.
There are four distinct ligase proteins found in mammalian cells. These DNA ligases are required for joining DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III is a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed, and complexes with XRCC-1 and a 97kDa (§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in base excision repair pathway.
Antigen: Full-length recombinant human DNA Ligase III-b.
Host Species: Mouse
Antibody Class: IgG1
Specificity: This antibody recognizes human, mouse, and chicken DNA Ligase III. Cellular localization is nuclear and mitochondrial.
Immunoblotting: use at 0.5-2ug/ml. Predicted molecular weight ~100kDa.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.