Anti-DNA Ligase I Antibody (56219)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,IHC-P,IP Human
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Overview
Product Name Anti-DNA Ligase I Antibody (56219)
Description Anti-DNA Ligase I Mouse Monoclonal Antibody
Target DNA Ligase I
Species Reactivity Human
Applications WB,ICC/IF,IHC-P,IP
Host Mouse
Clonality Monoclonal
Clone ID 10H5
Isotype IgG1
Immunogen Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human DNA Ligase I (125 kD), one of the four ligase proteins found in mammalian cells that are required for the joining of DNA strand breaks that occur during DNA replication, repair, and recombination.
Target Name DNA ligase 1
Target ID DNA Ligase I
Uniprot ID P18858
Alternative Names EC 6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1
Gene Name LIG1
Sequence Location Nucleus.
Biological Function DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Research Areas Cancer research
Application Images
Description Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] (56219) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA ligase I stained by DNA ligase I antibody [10H5] (56219) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 um.
Description DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DNA ligase I stained by DNA ligase I antibody [10H5] (56219) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5 ug/mL.

Immunoprecipitation: use at 1-5 ug/mL.

Positive controls: MCF-7 cells or recombinant protein.
References & Data Sheet