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Anti-Intercalated DNA Mouse Monoclonal Antibody – Library Pack (124111)

$265.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 2 x 100ug ELISA Eukaryotic
SKU: 124111 Categories: , , ,
Overview
Product Name Anti-Intercalated DNA Mouse Monoclonal Antibody - Library Pack (124111)
Description Anti-Intercalated DNA Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies:

12401 - Anti-Intercalated DNA Mouse Monoclonal Antibody - Clone ET602.2 - Isotype IgG2b

12402 - Anti-Intercalated DNA Mouse Monoclonal Antibody - Clone ET749.4 - Isotype IgG1

Target Intercalated DNA
Species Reactivity Eukaryotic
Applications ELISA
Host Mouse
Clonality Monoclonal
Immunogen Ethidium bromide intercalated calf thymus DNA.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity These antibodies specifically recognize intercalated eukaryotic DNA and do not cross-react with single or double-stranded non-intercalated DNA or with ethidium bromide.
Target ID Intercalated DNA
Research Areas DNA
Background Ethidium bromide (EB) found fame in the late 1940s as an antitrypanosomal, antimicrobial, antibacterial, and antiviral agent. Its biological effects are a direct consequence of the inhibition of nucleic acid synthesis, which in turn is related to the specific binding of the drug to DNA. EB inhibits DNA polymerase and binds in vitro to both RNA and DNA. Investigation into the precise nature of the DNA-EB binding mechanism led to the discovery that EB binds by a mechanism termed intercalation. This process has been studied extensively during the past three decades, and the photophysical changes that accompany intercalation have been successfully applied to quantitate and structurally elucidate DNA. More relevant to the use of EB in molecular biology are the observed variations in its fluorescent characteristics on binding to polynucleotides.
Application Images
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies may be used in ELISA to detect and quantitate intercalated DNA.
References & Data Sheet