Diacylglycerol ELISA Kit (ECP7839)
$718.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name Diacylglycerol ELISA Kit (ECP7839)
Description Diacylglycerol ELISA Kit (ECP7839) is a Competitive Inhibition ELISA for the quantitiation of Diacylglycerol in a variety of aqueous sample types.
Target Diacylglycerol
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 594.9 g·mol−1
Background Minimum Level of Detection: 153 pg/mL
Dynamic range of quantitation: 391-100,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 391-100,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Diacylglycerol Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Diacylglycerol Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 x 120uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| TMB Substrate | 1 x 9mL |
| Stop Solution | 1 x 6mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Diacylglycerol
Alternative Names 1-(hexadecanoyloxy)-3-hydroxypropan-2-yl-octadec-9-enoate, SCHEMBL638396, Glycerol 1-palmitate 2-oleate
PubChem ID 6026790
Research Areas Biochemicals
Background Diacylglycerol is a synthetic oil with anti-obesity activity. The enzymatically synthesized isoform, 1,3-isoform diacylglycerol, is suggested to decrease formation of chylomicrons as well as shunting them directly to the liver through the portal vein where they are oxidized. Increased beta-oxidation may enhance body weight loss, suppress body fat accumulation and lower serum triacylglycerol levels through increasing satiety.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.