DFF (CT)

Anti-DFF (CT) Antibody

ORDERING INFORMATION

Catalog No.: 2409
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.

BACKGROUND

A human DNA fragmentation factor (DFF) which is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death.

SPECIFICATION SUMMARY

Antigen: Peptide corresponding to aa 313-331 at the C-terminus of human DFF45 (accession no. NP_004392).
Host Speciec: Rabbit
Stabilizers: None
Preservatives: 0.02% sodium azide.

SPECIFICITY

This antibody recognizes non-cleaved (45kDa) and cleaved DFF45.

APPLICATIONS

Immunoblotting: use at 1ug/ml.
Immunocytochemistry: use at 5ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Positive control: Whole cell lysate from HeLa cells.

DILUTION INSTRUCTIONS

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE AND STABILITY

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.

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