Anti-DFF (CT) Antibody (2409)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IP,IF Human
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Overview
Product Name Anti-DFF (CT) Antibody (2409)
Description Anti-DFF (CT) BP Rabbit Polyclonal Antibody
Target DFF (CT)
Species Reactivity Human
Applications ELISA,WB,IHC-P,IP,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 313-331 at the C-terminus of human DFF45 (accession no. NP_004392).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes non-cleaved (45kDa) and cleaved DFF45.
Target Name DNA fragmentation factor subunitα
Target ID DFF (CT)
Uniprot ID O00273
Alternative Names DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD
Gene Name DFFA
Gene ID 1676
Accession Number NP_004392
Sequence Location Cytoplasm.
Biological Function Inhibitor of the caspase-activated DNase (DFF40).
Research Areas Apoptosis
Background A human DNA fragmentation factor (DFF) which is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death.
Application Images
Description Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution.
Description Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 ug/mL.
Description Immunofluorescence of DFF45 in Hela cells with DFF45 antibody at 20 ug/mL. Green: DFF45 Antibody (1409)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunocytochemistry: use at 5ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Whole cell lysate from HeLa cells.
References & Data Sheet