Anti-DAP Kinase 2 Antibody
Catalog No.: 2323
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is a pro- apoptotic, calcium-regulated serine/threonine kinase containing a death domain. Expression of DAPK induces cell death and suppresses oncogenic trans-formation. DAPK mediates IFN -induced apoptosis. A DAPK-related protein was recently described and designated DAPK2, or DRP-1. Expression of DAPK2 induces apoptosis. The messenger RNA for DAPK2 is expressed in multiple human tissues.
Antigen: Peptide corresponding to aa 356-370 of human DAPK2 (accession no. BAA88063). This sequence is identical to that of mouse.
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes human, mouse, and rat DAPK2 (approx. 42kDa) and does not cross- react with DAPK.
Immunoblotting: use at 1ug/ml.
Immunohistochemistry: use at 2ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Positive control: Whole cell lysate from A431cells.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic applications.