Anti-Cytokeratin 18 Antibody (34058)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ul IHC Human
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Overview
Product Name Anti-Cytokeratin 18 Antibody (34058)
Description Anti-Cytokeratin 18 Mouse Monoclonal Antibody
Target Cytokeratin 18
Species Reactivity Human
Applications IHC
Host Mouse
Clonality Monoclonal
Clone ID G018.1
Isotype IgG1
Immunogen Recombinant human Cytokeratin 18.
Properties
Form Liquid
Concentration Lot Specific
Formulation Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
Buffer Formulation Tris
Buffer pH pH 7.3-7.7
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity Human Cytokeratin 18. Reactivity with other species has not been investigated.
Target Name Keratin, type I cytoskeletal 18
Target ID Cytokeratin 18
Uniprot ID P05783
Alternative Names Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18
Gene Name KRT18
Sequence Location Cytoplasm, perinuclear region. Nucleus, nucleolus.
Biological Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. {PubMed:15529338, PubMed:16424149, PubMed:17213200, PubMed:7523419, PubMed:8522591, PubMed:9298992, PubMed:9524113}.
Research Areas Cancer research
Background Cytokeratin 18 is a type I cytokeratin. Together with its filament partner cytokeratin 8, it is perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues. Mutations in this gene have been linked to cryptogenic cirrhosis. Multimers of cytokeratin 18 and cytokeratin 8 are present in simple, glandular, and transitional epithelial cells, but absent from stratified epithelial cells, and are found in adenocarcinomas of simple and glandular epithelium origin and poorly differentiated squamous carcinoma cells.
Application Images
Description Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes. Detection of Cytokeratin 18 in human breast carcinoma with #34058 diluted 1:100-1:200.
Additional Information
Handling
Storage Store at 2-8°C. Do not freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
References & Data Sheet