Corticotropin-Releasing Factor Monoclonal Antibody
Catalog No.: 56101 (4H9) and 56102 (5D5).
Size: 100 ug purified antibody in PBS, pH 7.4.
Antigen: Purified human/rat corticotropin-releasing factor (CRF, Sigma).
Host Species: Mouse
Antibody Class: IgG1
Purification method: Three sequential precipitations with 40% ammonium sulfate. Purified antibodies yield 1 band in native SDS-PAGE.
Preservatives: None. Available on request.
These antibodies react specifically with CRF. There is no cross-reactivity with bovine serum albumin, insulin, ovalbumin, or epidermal growth factor. These two antibodies recognize different epitopes on CRF. Dissociation constants = 10-9 M for 56101and 1.5 x 10-8 M for 56102.
These antibodies may be used in immunoassays to detect and quantitate CRF. Other applications are under investigation.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Kravchenko and Furalev. 1994. Hybridoma 13: 59-64;
Furalev et al. 1994. Biochem Biophys Res Com 204: 823-834.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedure