Anti-Collagen Vα-1 Chain N-propeptide-NC2 domain Antibody (90008)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC Human
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SKU: 90008 Categories: , ,
Overview
Product Name Anti-Collagen Vα-1 Chain N-propeptide-NC2 domain Antibody (90008)
Description Anti-Collagen Vα-1 Chain (NC2 domain) Mouse Monoclonal Antibody
Target Collagen Vα-1 Chain N-propeptide-NC2 domain
Species Reactivity Human
Applications WB,IHC
Host Mouse
Clonality Monoclonal
Clone ID 18G5
Isotype IgG1
Immunogen Recombinant human collagen V alpha-1 chain N fragment.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody reacts with human collagen V alpha-1 chain propeptide. The epitope recognized is in the COL2- NC2 region. Cross-reactivity with mouse is predicted based on sequence homology.
Target Name Collagenα-1(V) chain
Target ID Collagen Vα-1 Chain N-propeptide-NC2 domain
Uniprot ID P20908
Gene Name COL5A1
Sequence Location Secreted, extracellular space, extracellular matrix
Biological Function Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Research Areas Cell adhesion
Background Collagen V alpha-1chain is a protein that in humans is encoded by the COL5A1 gene. This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
Application Images
Description Immunohistochemistry: Formalin-fixed, paraffin-embedded tissue, heat-induced antigen retrieval. Use at 1-10ug/ml. Staining of human skin with #90008.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions Immunoblotting: use at 1-5ug/mL.

Immunohistochemistry: Formalin-fixed, paraffin-embedded tissue, heat-induced antigen retrieval. Use at 1-10ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet