Anti-Collagen Type IV Antibody

$229.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ul IHC Human
SKU: 34056 Categories: , , Tag:
Overview
Product Name Anti-Collagen Type IV Antibody (34056)
Description Anti-Collagen Type IV Mouse Monoclonal Antibody
Target Collagen Type IV
Species Reactivity Human
Applications IHC
Host Mouse
Clonality Monoclonal
Clone ID G549.1
Isotype IgG1
Immunogen Recombinant human Collagen Type IV.
Properties
Form Liquid
Concentration Lot Specific
Formulation Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
Buffer Formulation Tris
Buffer pH pH 7.3-7.7
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity Human Collagen Type IV. Reactivity with other species has not been investigated.
Target Name Collagenα-1(IV) chain [Cleaved into: Arresten]
Target ID Collagen Type IV
Uniprot ID P02462
Gene Name COL4A1
Sequence Location Secreted, extracellular space, extracellular matrix, basement membrane
Biological Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. {UniProtKB:P02463}.; Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. {PubMed:10811134, PubMed:18775695}.
Research Areas Cancer research
Background Type IV collagen is the main collagen component of the basement membrane. It is a network- forming collagen that underlies epithelial and endothelial cells and functions as a barrier between tissue compartments. Each type IV collagen molecule is a heterotrimer composed of three alpha chains. Type IV collagen triple helices form open, nonfibrillar networks that associate with laminin assemblies.
Application Images
Description Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes. Detection of Collagen Type IV in human lung with #34056 diluted 1:100-1:200.
Additional Information
Handling
Storage Store at 2 - 8°C. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
References & Data Sheet