Clostridium difficile Toxin A Monoclonal Antibody
Catalog No.: 18910
Clone No.: PCG4
MAb Subtype: IgG2a
Format: 1mg/ml Protein G-purified antibody in PBS, pH 7.2, 0.1% sodium azide.
Clostridium difficile is a Gram-positive anaerobic bacterium that is the major causative agent of colitis and diarrhea primarily in hospitalized patients who have undergone antibiotic therapy. Pathogenic C. difficile strains produce multiple toxins. The best characterized are Toxin A and Toxin B, both of which may produce diarrhea and inflammation in infected patients. Toxins A and B are glucosyltransferases that target and inactivate the Rho family of GTPases. C. difficile most commonly affects older adults in hospitals or in long-term care facilities. However, studies show increasing rates of C. difficile infection among people traditionally not considered high risk, such as younger and healthy individuals without a history of antibiotic use or exposure to health care facilities.
Antigen: Toxin A purified from culture filtrates of C. difficile 10463 and inactivated with 0.4% formalin.
Accession no.: AA23283
Gene ID: 144926
Host Species: Mouse
Specificity: This antibody recognizes C. difficile Toxin A. It does not cross-react with Toxin B.
ELISA: for detection use at 1-10ug/ml.
Immunoblotting: use at 0.5-2ug/ml.
Immunofluorescence: use at 1-5ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20o to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.