Clostridium botulinum Toxin D Monoclonal Antibody
Catalog No.: 18915
Clone No.: 5131
MAb Subtype: IgG1
Format: Purified antibody in PBS, pH 7.2, 0.1% sodium azide.
Clostridium botulinum, an anaerobic, gram-positive, spore-forming rod commonly found on plants, in soil, water, and the intestinal tracts of animals, produces eight antigenically distinguishable exotoxins (A, B, C1, C2, D, E, F and G). Type A is the most potent toxin, followed by types B and F. All botulinum neurotoxins are produced as single polypeptide chains of ~150kDa comprised of a heavy (H) chain and a light (L) chain of roughly 100 and 50kDa, respectively, linked by a disulfide bond. The heavy (H) chain of the toxin binds selectively and irreversibly to high affinity receptors at the presynaptic surface of cholinergic neurones, and the toxin-receptor complex is taken up into the cell by endocytosis where the disulfide bond between the two chains is cleaved. The light (L) chain interacts with different proteins in the nerve terminals to prevent fusion of acetylcholine vesicles with the cell membrane.
Antigen: Toxin D purified from culture filtrate of C. botulinum.
Host Species: Mouse
Accession no.: P19321
Specificity: This antibody recognizes C. botulinum Toxin D.
ELISA: use a dilution of 1:20-1:200. Immunocytochemistry/Immunohistochemistry: use a dilution of 1:10-1:50. Immunofluorescence: use a dilution of 1:10-1:50.
Immunoblotting: use a dilution of 1:100-1:1,000. A band of ~150,000 is detected.
Endusers should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable at 4°C for short-term storage and for at least one (1) year at -20o to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.