Anti-human CIDE-3 Antibody
Catalog No.: 11093
Size: 100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma- tography.
DNA fragmentation, induced by DNA fragmentation factor (DFF), is one of the critical steps in apoptosis. DFF is composed of two subunits, a 40-kDa caspase-activated nuclease (DFF40) and a 45-kDa inhibitor (DFF45). Recently a novel family of cell death- inducing DFF45-like effectors (CIDEs) has been identified. Human CIDE-3 is a novel CIDE. Two transcripts, CIDE-3 and CIDE-3alpha, are present in HepG2 and A375 cells. Consistent with its chromosome localization at 3p25, a region associated with high frequency loss of heterozygosity in many tumors, CIDE-3 may play an important role in prevention of tumorigenesis.
Liang, L et al. (2003) Biochem J 370: 195-203.
Immunogen: Synthetic peptide corresponding to amino acids 10-25 (LLYPKSLSRHVSVRTS) of human CIDE-3 conjugated to KLH at the C- terminus.
Host Species: Rabbit
Preservatives: None. Available on request.
This antibody recognizes human CIDE-3. Sequence homology to the mouse CIDE-3 homolog, FSP27, is 93%.
Immunoblotting: use at 1:500 dilution. A band of approximately 27 kDa is detected.
Positive control: HepG2 cell lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.