Anti-Chromogranin B Antibody

$516.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB Human, Mouse, Rat
SKU: 26108 Categories: , ,
Overview
Product Name Anti-Chromogranin B Antibody (26108)
Description Anti-Chromogranin B Rabbit Polyclonal Antibody
Target Chromogranin B
Species Reactivity Human, Mouse, Rat
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide derived from the C-terminus (aa 641-655) of CgB (accession no. NP_001810. This sequence is identical in human, mouse, and rat CgB.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH 7.4
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat chromogranin B.
Target Name Secretogranin-1
Target ID Chromogranin B
Uniprot ID P05060
Alternative Names Chromogranin-B, CgB, Secretogranin I, SgI [Cleaved into: PE-11; GAWK peptide; CCB peptide]
Gene Name CHGB
Sequence Location Secreted. Note=Neuroendocrine and endocrine secretory granules.
Biological Function Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Research Areas Hormones/Proteins
Background Chromogranin B (CgB, secretogranin I) is a secretory granule matrix protein expressed in a wide variety of endocrine cells and neurons where it may have both extracellular and intracellular functions. Extracellular roles for CgB are dependent on its proteolytic processing within secretory granules to form smaller peptides that may have a role in the neuroendocrine/sympathoadrenal stress response to systemic infection, and CgB fragments may also have an autocrine inhibitory role in the release of co-stored hormones, such as insulin.
Application Images
Description mmunoblotting: use at 0.1-1.0ug/ml PC12 cell extract (ug) 2.5 1.0 Although the predicted mw of CgB is ~76kDa, the highly acidic nature of CgB causes it to run at a higher mw in SDS-PAGE.
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.1-1.0ug/mL

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.Although the predicted mw of CgB is ~76kDa, the highly acidic nature of CgB causes it to run at a higher mw in SDS-PAGE.

Immunohistochemistry: use at 1-5ug/mL.
References & Data Sheet
References Thompson ME et al. 1992. Prolactin granulogenesis is associated with increased secretogranin expression and aggregation in the Golgi apparatus of Gh4C1 cells. Endocrinology 131: 318-326.
PMID 1612012