ch-TOG Polyclonal Antibody
Catalog No.: 34032
Size: 100ul antiserum
Antigen: NH2 terminal cysteine-peptide corresponding to aa 2019-2032 in the carboxyl terminal region of human ch- TOG coupled to KLH: NH2-CDLKKRLERIKSSRK-COOH.
Host Species: Rabbit
Antibody Class: Polyclonal
Preservatives: None. Available on request.
Colonic and hepatic tumor over-expressed protein (ch-TOG) belongs to the TOG/XMAP215/Dis1 evolutionarily ancient family of microtubule-associated proteins with isoforms found in all major kingdoms of eukaryotes. This protein is required for the assembly and function of the meiotic or mitotic spindles during cell division and is localized to microtubule-organizing centers (MTOCs) during interphase or to the spindle poles and centrosomes during mitosis or cytokinesis.
This antibody recognizes human and mouse ch-TOG. A BLAST search demonstrated 100% identity to the peptide immunogen in human, mouse, rat, chimpanzee, dog, cow, chicken, opossum, and frog.
Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of approx. 215kDa is detected.
Immunoprecipitation: use at 1:5,000- 1:10,000 dilution.
Immunofluorescence: use at 1:5,000- 1:10,000 dilution.
These are recommended concentrations. User should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.