CENP-A Monoclonal Antibody
Catalog No.: 12597 (clone 5A7-2E11)
Format: 100ug in PBS (1mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Replicated chromosomes include two kinetochores that control chromosome segregation during mitosis. Centromere Protein-A (CENP-A), a histone H3-like protein, contains a C-terminal H3-like domain which is required for centromere localization of CENP-A. It is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H2A, H2B, and H4. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on chromosomes.
Antigen: Synthetic peptide corresponding to a sequence within human CENP-A.
Accession no.: P49450, NP_001035891.1
Host Species: Mouse
Antibody Class: IgG1
Specificity: This antibody recognizes human CENP-A. Reactivity with other species has not been investigated.
Immunoblotting: use at 1ug/ml. A band of ~18kDa is detected.
Detection of CENP-A in lysate of U2OS cells
ELISA: use at 1-10ug/ml with CENP-A on the solid phase.
Positive control: U2OS cell lysate
These are recommended concentrations; enduser should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.