Anti-CDC25a Antibody

$503.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	100ug	WB,IP	Human

SKU: 13022 Categories: Antibody Products, Enzymes and Enzyme Inhibitor Antibodies, Products

Overview

Product Name Anti-CDC25a Antibody (13022)

Description Anti-CDC25a Rabbit Polyclonal Antibody

Target CDC25a

Species Reactivity Human

Applications WB,IP

Host Rabbit

Clonality Polyclonal

Isotype Whole IgG

Immunogen Synthetic peptide representing a portion of the protein encoded by exon 7 (LocusLink ID 993).

Properties

Form Liquid

Concentration Lot Specific

Formulation Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Buffer Formulation Tris

Buffer pH pH 7.8

Buffer Anti-Microbial 0.1% Sodium Azide

Format Purified

Purification Purified by Protein A affinity chromatography

Specificity This antibody reacts with human. Other species have not been tested. Antibody is affinity purified.

Target Name M-phase inducer phosphatase 1

Target ID CDC25a

Uniprot ID P30304

Alternative Names EC 3.1.3.48, Dual specificity phosphatase Cdc25A

Gene Name CDC25A

Accession Number NP_001780

Biological Function Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Research Areas Enzymes

Description Detection of human CDC25a by western blot of immunoprecipitates. Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody 13022 (lot 13022-2) used for IP at 6 µg per reaction. CDC25a was also immunoprecipitated by rabbit anti-CDC25a antibody. For blotting immunoprecipitated CDC25a, 13022 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Description Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody 13022 (lot 13022-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Handling

Storage Store at 2 - 8ºC. Do not Freeze.

Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.

Application Instructions Western Blot: 0.1 - 1 ug/ml.

Immunoprecipitation: 1- 4 ug/mg of lysate.

Data Sheet