Anti-CDC25a Antibody (13022)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IP Human
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SKU: 13022 Categories: , ,
Overview
Product Name Anti-CDC25a Antibody (13022)
Description Anti-CDC25a Rabbit Polyclonal Antibody
Target CDC25a
Species Reactivity Human
Applications WB,IP
Host Rabbit
Clonality Polyclonal
Isotype Whole IgG
Immunogen Synthetic peptide representing a portion of the protein encoded by exon 7 (LocusLink ID 993).
Properties
Form Liquid
Concentration Lot Specific
Formulation Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Buffer Formulation Tris
Buffer pH pH 7.8
Buffer Anti-Microbial 0.1% Sodium Azide
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody reacts with human. Other species have not been tested. Antibody is affinity purified.
Target Name M-phase inducer phosphatase 1
Target ID CDC25a
Uniprot ID P30304
Alternative Names EC 3.1.3.48, Dual specificity phosphatase Cdc25A
Gene Name CDC25A
Accession Number NP_001780
Biological Function Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Research Areas Enzymes
Application Images
Description Detection of human CDC25a by western blot of immunoprecipitates. Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody 13022 (lot 13022-2) used for IP at 6 µg per reaction. CDC25a was also immunoprecipitated by rabbit anti-CDC25a antibody. For blotting immunoprecipitated CDC25a, 13022 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Description Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody 13022 (lot 13022-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Additional Information
Handling
Storage Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Western Blot: 0.1 - 1 ug/ml.

Immunoprecipitation: 1- 4 ug/mg of lysate.
References & Data Sheet