Anti-Cav3.2 Ca+2 Channel Monoclonal Antibody
Catalog No.: 11505 (clone S55-10)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Cav3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest that certain mutations in this gene lead to childhood absence epilepsy. It has also been suggested that up-regulation of Cav3.2 may be involved in the progression of prostate cancer toward an androgen -independent stage.
Antigen: Fusion protein corresponding to aa 1019-1293 (II-III loop)
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human, mouse, and rat Cav3.2. It does not cross-react with Cav1.3.
Immunoblotting: use at 1-10ug/ml. A band of >260kDa is detected.
Immunohistochemistry and and immunocytochemistry: use at 0.1-1ug/ml.
Immunofluorescence: use at 1-10ug/ml.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain membranes.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.