Anti-Cavβ4 Ca+2 Channel Monoclonal Antibody
Catalog No.: 11501 (clone S10-7)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Calcium channel, voltage-dependent, beta-4 subunit, also known as CACNB4, belongs to the calcium channel beta subunit family. This protein plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting, and shifting the voltage dependence of activation and inactivation. Mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Alternate transcriptional splice variants of this gene, encoding different isoforms, have been identified.
Antigen: Synthetic peptide corresponding to aa 458-474 of rat Cav54.
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human, mouse, and rat Cavβ4. It does not cross-react with Cav β1b, Cav β2a, or Cav β3.
Immunoblotting: use at 1-10ug/ml. A band of ~50kDa is detected.
Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml
Immunofluorescence: use at 1-10ug/ml
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain lysate
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.