Anti-Caspase-9 (I20) Antibody
Catalog No.: 2418
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3, a protease responsible for proteolytic cleavage of many key proteins involved in apoptosis. Caspase-9 plays a central role in cell death induced by a wide variety of apoptosis induced by a wide variety of apoptosis inducers including TNF , TRAIL, anti-CD95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Immunoblotting: use at 1ug/ml.
Immunocytochemistry: use at 2ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Positive control: Whole cell lysate from HeLa cells.
Antigen: Peptide corresponding to aa 299- 318 of human caspase-9 (accession no. P55211).
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes full-length human caspase-9 (46kDa).
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.