Caspase-3 Polyclonal Antibody
Catalog nos.: 11024-50 (50ug) 11024-200 (200ug)
Format: Protein A-purified antibody in PBS, 50% glycerol, 0.09% sodium azide.
Caspases are cysteine proteases that cleave after certain aspartate residues and have been identified as mediators of apoptosis. Caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.
Antigen: Recombinant full length human caspase-3.
Accession no.: P42574
Gene ID: 836
Host Species: Rabbit
Antibody Class: Polyclonal
Specificity: This antibody recognizes caspase-3 in human, mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, pig, rabbit, and yeast.
Immunoblotting: use at 1-5ug/ml. A band of ~36kDa is detected.
Immunohistochemistry: use at 10ug/ml
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This product is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for therapeutic or diagnostic procedures.