Anti-Caspase-13 Antibody (2245)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P Human, Mouse, Rat
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Overview
Product Name Anti-Caspase-13 Antibody (2245)
Description Anti-Caspase-13 Rabbit Polyclonal Antibody
Target Caspase-13
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 91- 105 of human caspase-13 (accession no. O75601).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat caspase-13 (43kDa).
Target Name Caspase-13
Target ID Caspase-13
Uniprot ID O75601
Alternative Names CASP-13, EC 3.4.22.-, Evolutionary related interleukin-1-β-converting enzyme, ERICE [Cleaved into: Caspase-13 subunit 1; Caspase-13 subunit 2]
Gene Name CASP13
Gene ID 338039
Accession Number NP_788811.1
Biological Function Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
Research Areas Apoptosis
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. Caspase-13 or ERICE (Evolutionarily Related Interleukin-1 Converting Enzyme) was recently described. When activated by caspase-8, caspase-13 induces apoptosis. Caspase-13 is expressed in a variety of human tissues and cell lines.
Application Images
Description Western blot analysis of caspase-13 in human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 ug/mL.
Description Immunohistochemistry of caspase-13 in mouse brain tissue with caspase-13 antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunohistochemistry: use at 10ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.

Positive control: Whole cell lysate from HL60 cells.
References & Data Sheet