Anti-Caspase-10 (CT) Antibody (2408)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IF,ICC Human
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Overview
Product Name Anti-Caspase-10 (CT) Antibody (2408)
Description Anti-Caspase-10 (CT) Rabbit Polyclonal Antibody
Target Caspase-10 (CT)
Species Reactivity Human
Applications ELISA,WB,IF,ICC
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 505-521 at C-terminus of human FLICE2 (accession no. AAD28402).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length human caspase-10 (59kDa). Since the sequences at C- termini of FLICE2 and Mch4 are different, this antibody recognizes only the FLICE2 form of caspase-10.
Target Name Caspase-10
Target ID Caspase-10 (CT)
Uniprot ID Q92851
Alternative Names CASP-10, EC 3.4.22.63, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2, ICE-like apoptotic protease 4 [Cleaved into: Caspase-10 subunit p23/17; Caspase-10 subunit p12]
Gene Name CASP10
Gene ID 843
Accession Number NP_116756
Biological Function Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. {PubMed:11717445}.; Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis. {PubMed:17822854}.; Isoform C is proteolytically inactive. {PubMed:11717445}.
Research Areas Apoptosis
Background A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4, and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is, therefore, involved in CD95- and TNFR1-induced apoptosis. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8, and -9, thereby causing proteolytic cleavage of many key proteins, such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase- 10 is expressed in many tissues and cell lines.
Application Images
Description Western blot analysis of caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody at 1 ug/mL.
Description Immunofluorescence of Casp10 in Hela cells with Caspase 10 antibody at 20 ug/mL. Green: Caspase-10 Antibody (2408)
Blue: DAPI staining
Description Immunocytochemistry of Casp10 in Hela cells with Caspase 10 antibody at 5 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Whole cell lysate from HeLa cells.
References & Data Sheet