Anti-Caspase-10 (CT) Antibody
Catalog No.: 2408
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4, and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is, therefore, involved in CD95- and TNFR1-induced apoptosis. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8, and -9, thereby causing proteolytic cleavage of many key proteins, such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase- 10 is expressed in many tissues and cell lines.
Antigen: Peptide corresponding to aa 505-521 at C-terminus of human FLICE2 (accession no. AAD28402).
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
Immunoblotting: use at 1ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Positive control: Whole cell lysate from HeLa cells.
This antibody recognizes full-length human caspase-10 (59kDa). Since the sequences at C- termini of FLICE2 and Mch4 are different, this antibody recognizes only the FLICE2 form of caspase-10.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.