Overview
Product Name Carnitine ELISA Kit (ECP7826)
Description Carnitine ELISA Kit (ECP7826) is a Competitive Inhibition ELISA for the quantitiation of Carnitine in a variety of aqueous sample types.
Target Carnitine
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 492.4 g·mol−1
Background Minimum Level of Detection: 5.04 ng/mL
Dynamic range of quantitation: 12.35-1,000 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 12.35-1,000 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
Reagents | Quantity |
Carnitine Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
Adhesive Plate Seal | 4 Each |
Carnitine Standard | 2 Vials |
Standard Diluent | 1 x 20mL |
Detection Reagent A | 1 x 120uL |
Diluent A | 1 x 12mL |
Detection Reagent B | 1 x 120uL |
Diluent B | 1 x 12mL |
TMB Substrate | 1 x 9mL |
Stop Solution | 1 x 6mL |
30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Carnitine
Alternative Names Cochineal, 1343-78-8, 1390-65-4, 3,5,6,8-tetrahydroxy-1-methyl-9,10-dioxo-7-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]anthracene-2-carboxylic acid
CAS Number 1343-78-8
PubChem ID 14950
Research Areas Biochemicals
Background Red food colouring The source of this color is a picture of a "deep carmine pink" flower at the following website:; The source of this color is a watercolor color swatch called light carmine displayed at the following website:. Carmine red belongs to the family of Anthraquinone Glycosides. These are organic compounds containing an anthraquinone moiety glycosidically bound to a carbohydrate moiety.
Application Images

Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.