Anti-Canine PD-L1

$447.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug FACS,Inhibition,IHC Canine
SKU: 34091 Categories: , ,
Overview
Product Name Anti-Canine PD-L1 (34091)
Description Anti-Canine PD-L1 Mouse Monoclonal Antibody
Target Canine PD-L1
Species Reactivity Canine
Applications FACS,Inhibition,IHC
Host Mouse
Clonality Monoclonal
Clone ID JC071
Immunogen Recombinant extracellular domain of canine PD-L1 fused with the Fc domain of human IgG1 expressed in D. melanogaster S2 cells.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes canine PD-L1.
Target ID Canine PD-L1
Application Images
Description Detection of PD-L1 expression on CLGL-90 cells (Chronic Large Granular Lymphocytic-90) stained with JC071 (dark gray) vs. mouse IgA isotype control (light gray). Endusers should determine optimal antibody concentrations for their applications.
Description JC071 antibody added to ELISA plates coated with PD-L1 followed by the addition of biotinylated PD-1 and streptavidin-HRP. JC071 antibody blocked binding of PD-1 to PD-L1 as compared with binding of PD-1 to PD-L1 in the presence of culture medium or PBS.
Additional Information
Handling
Storage Store at 2 - 8°C. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Flow cytometry Detection of PD-L1 expression on CLGL-90 cells (Chronic Large Granular Lymphocytic-90) stained with JC071 (dark gray) vs. mouse IgA isotype control (light gray).

Endusers should determine optimal antibody concentrations for their applications. Inhibition of PD-1 and PD-L1 Interaction JC071 antibody added to ELISA plates coated with PD-L1 followed by the addition of biotinylated PD-1 and streptavidin-HRP. JC071 antibody blocked binding of PD-1 to PD-L1 as compared with binding of PD-1 to PD-L1 in the presence of culture medium or PBS.
References & Data Sheet