Anti-Canine PD-1 (34090)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 100ug | IHC,FACS,Inhibition | Canine |  |
Overview
Product Name Anti-Canine PD-1 (34090)
Description Anti-Canine PD-1 Mouse Monoclonal Antibody
Target Canine PD-1
Species Reactivity Canine
Applications IHC,FACS,Inhibition
Host Mouse
Clonality Monoclonal
Clone ID JC053
Isotype IgA
Immunogen Recombinant extracellular domain of canine PD-1 fused with the Fc domain of human IgG1 expressed in D. melanogaster S2 cells.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification PEG Purified
Specificity Information
Specificity This antibody recognizes canine PD-1.
Target ID Canine PD-1
Handling
Storage This antibody is stable for at least one (1) year at -20°C to-70°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Canine PD-1 Monoclonal Antibody Flow cytometry Detection of PD-1 expression on CLGL-90 cells (Chronic Large Granular Lymphocytic-90) stained with JC053 (dark gray) vs. mouse IgA isotype control (light gray). Inhibition of PD-1 and PD-L1 Interaction JC053 antibody added to ELISA plates coated with PD-1 followed by the addition of biotinylated PD-L1and streptavidin-HRP. JC053 antibody blocked binding of PD-1 to PD-L1 as compared with binding of PD-1 to PD-L1 in the presence of a non-inhibitory antibody 73H2.
End users should determine optimal antibody concentrations for their applications.
End users should determine optimal antibody concentrations for their applications.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet