Canine PD-1

 Canine PD-1 Monoclonal Antibody 

ORDERING INFORMATION 

Catalog No. Clone No. MAb Subtype Size 

34090 JC053 IgA 100ug 

Format: PEG-purified antibody in PBS, pH 7.4. Concentration is lot-specific; please refer to the vial label. 

BACKGROUND 

Each year, thousands of dogs are diagnosed with cancer. Canine cancers bear many of the hallmarks of human cancers including tissue location, tumor progression, and response to chemotherapy and radiation. Unfortunately, canine lymphocyte populations are not as well defined as human lymphocytes, and reagents that target immune checkpoint pathways are not yet widely available for veterinary research. Interruption of the programmed death 1 (PD-1) / programmed death ligand 1 (PD-L1) pathway is an established and effective therapeutic strategy in human oncology and may be effective for treating canine cancers as well. 

SPECIFICATION SUMMARY 

Antigen: Recombinant extracellular domain of canine PD-1 fused with the Fc domain of human IgG1 expressed in D. melanogaster S2 cells. 

Host Species: Mouse 

Specificity: This antibody recognizes canine PD-1. 

APPLICATIONS 

Immunohistochemistry 

IgA control JC053 

CHO-PD1 cells fixed with 10% neutral buffered formalin, prepared in Histogel and embedded in paraffin, antigen retrieval in citrate buffer at pH 6. Cells stained with IgA isotype control or with JC053. 

Tonsil, IgA control Tonsil, JC053 Lymph node, IgA control Lymph node, JC053 

Formalin-fixed, paraffin-embedded canine tonsil and lymph node stained with IgA isotype control or with JC053. 

Canine PD-1 Monoclonal Antibody 

Flow cytometry 

Detection of PD-1 expression on CLGL-90 cells (Chronic Large Granular Lymphocytic-90) stained with JC053 (dark gray) vs. mouse IgA isotype control (light gray). 

Inhibition of PD-1 and PD-L1 Interaction 

JC053 antibody added to ELISA plates coated with PD-1 followed by the addition of biotinylated PD-L1and streptavidin-HRP. JC053 antibody blocked binding of PD-1 to PD-L1 as compared with binding of PD-1 to PD-L1 in the presence of a non-inhibitory antibody 73H2. 

Endusers should determine optimal antibody concentrations for their applications. 

All images courtesy of Dr. JW Choi, University of California, Davis. 

STORAGE AND STABILITY 

This antibody is stable for at least one (1) year at -20°C to-70°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles. 

PRODUCT REFERENCE 

Choi JW et al. 2020 Development of canine PD-1/PD-L1 specific monoclonal antibodies and amplification of canine T cell function. PLoS One. 2020; 15(7): e0235518. 

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. 

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