Anti-Calreticulin Polyclonal Antibody
Catalog No.: 56259
Size: 100ul whole antiserum
Calreticulin is a multifunctional highly conserved Ca+2-binding protein that is localized primarily in the endoplasmic reticulum (ER) but has also been detected in the nucleus and nuclear envelope. Like many other ER proteins, it has the conserved ER retention KDEL sequence (Lys-Asp-Glu-Leu) at its C-terminus. Calreticulin is made up of three domains: 1 180 residue N-terminal domain, a proline- rich P-domain (aa 189-288) that binds Ca+2 with high affinity and shares homology with calnexin and calmegin, and a 110 residue C-terminal domain that binds Ca+2 with low affinity but high capacity. Calreticulin appears to be involved in calcium storage and regulation, has molecular chaperone activity, and has been shown to be induced in response to various types of cell stress.
Antigen: Human calreticulin synthetic peptide conjugated to KLH.
Host Species: Rabbit
Antibody Class: Polyclonal
This antibody recognizes human, mouse, rat, bovine, canine, chicken, guinea pig, monkey, pig, hamster, rabbit, and sheep calreticulin.
Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of ~63 kDa is detected.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.