Calnexin Monoclonal Antibody
Catalog No.: 90007 (clone C2A1)
Format: 100ug, Protein A-purified, lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Calnexin is a ~90 kDa integral protein of the endoplasmic reticulum; it is also referred to as IP90, p88 and p90. It consists of a 50kDa N-terminal calcium- binding luminal domain, a single transmembrane helix, and a short acidic cytoplasmic tail. Its positively charged cytosolic residues ensure its retention in the endoplasmic reticulum. Calnexin together with calreticulin play a key role in glycoprotein folding by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor, and B cell surface immunoglobulin.
Antigen: Dictyostelium discoideum AX2 recombinant fragment. Accession no. P27824.
Host species: Mouse.
Antibody Class: IgG1.
This antibody reacts with human and Dictyostelium discoideum calnexin.
Immunoblotting: use at 1-10ug/ml.
Immunohistochemistry: use at 1-10ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Reconstitute in distilled water.
STORAGE AND STABILITY
This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.