c-myc Affinity-Purified Polyclonal Antibody, HRP conjugate
Catalog No., Size
Format: 1mg/ml conjugated to horseradish peroxidase (HRP) in Phosphate Buffered Saline, pH 7.2, 0.2% BSA, 0.1% Pro-Clean 400. Molar enzyme/antibody ratio = 4:1.
c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids.
Antigen: Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.
Host Species: Rabbit
Specificity: Antibody was affinity-purified on the peptide.
Immunoblotting: use at a dilution of 1:1,000- 1:30,000.
Immunocytochemistry: use at a dilution of 1:200-1:500.
ELISA: use at a dilution of 1:10,000- 1:100,000.
These are recommended dilutions. Endusers should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at 2-8°C.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.