Awaiting product image

Anti-c-Jun (Phospho-Tyr170) Antibody (43023)

$366.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB Human, Mouse, Rat
SKU: 43023 Categories: ,
Overview
Product Name Anti-c-Jun (Phospho-Tyr170) Antibody (43023)
Description Anti-c-Jun (Phospho-Tyr170) Rabbit Polyclonal Antibody
Target c-Jun (Phospho-Tyr170)
Species Reactivity Human, Mouse, Rat
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of tyrosine 170 (P-V-Y(p)-A-N) derived from human c-Jun and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat c-Jun only when phosphorylated at tyrosine 170.
Target ID c-Jun (Phospho-Tyr170)
Post Translational Modification c-Jun (Phospho-Tyr170)
Target Phospho-tyr170
Research Areas Phosphospecific Antibodies
Background c-Jun in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet